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"I've tried to do things right for my club, and it's well done to Southampton," added the Blackpool boss.
As well as making nine changes, Holloway used half of Blackpool's youth team to make up the bench for the 1953 FA Cup winners.
"I thoroughly enjoyed it, it's very exciting to be able to give these young lads a chance," said Holloway, whose side are playing in the Premier League for the first time after winning promotion following their play-off win over Cardiff last season.
I hope this will in some way wake Oyston up in his deluded belief that he can revolutionise the whole of the British football transfer system at Blackpool.
"Obviously I didn't bring the team that has been playing in the Premier League for the rest of the season due to the size of my squad.
"To bring all my second-year YTS players along and give them a chance, it's a great day for them."
If Holloway was gracious in his praise of Southampton's win, he was less than impressed with some of the Saints fans' chants.
"It's an unbelievable turnaround to have their fans singing 'Premier League, you're having a laugh' at us," added the Blackpool manager.
"Well I am having a laugh, I am loving every minute of it because my team has earned the right to be there.
"I don't think they should be quite so disrespectful, maybe they're a little bit jealous of the fact a little club like Blackpool can do it and Southampton have fallen from grace. But I'm sure they'll be back."
A delighted Saints boss Nigel Adkins is hoping to face Liverpool in the next round - if new caretaker manager manager Kenny Dalglish and his side beat Manchester United at Old Trafford on Sunday.
"I've always said I wanted Liverpool in the cup from day one," said Adkins.
"Blackpool have always been a bogey club for me so it is fantastic to break that bogey.
"Is it an upset? We made eight changes, we both utilised our squads. We should have scored more goals than we did."